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Reaching the sufficient range (20 ng/mL or 50 nmol/L) is the threshold used in the PCOS-fertility meta-analysis above, 4 though some endocrine guidelines target 30 ng/mL (75 nmol/L) for general health

PPA exposure produced pronounced oxidative stress and disruptions in lipid metabolism, whereas post-treatment with omega-3 or vitamin B12, administered individually or together, partially normalized antioxidant defenses, modulated inflammatory enzyme activity, and altered microbial profiles

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Then, 1 mL of the overnight culture was transferred into 50 mL of LB or M9 medium in 250 mL flasks for further cultivation (kanamycin or ampicillin was added when needed)